Transcriptome mining, functional characterization, and phylogeny of a large terpene synthase gene family in spruce (Piceaspp.)

Table 1 In silico identification of TPSs in the EST databases of Sitka spruce, white spruce, and hybrid white spruce

Spruce Species	Total ESTs	Total Singlets Plus Contigs	TPS ESTs*	TPS Singlets	TPS Contigs	Total TPSs
White	242,931	59,449	181	36	33	69
Sitka	174,384	37,533	282	25	30	55
Hybrid White	26,350	13,279	43	10	10	20

*Conifer TPS protein sequences available from NCBI were used to query the three species-specific EST databases using the tBLASTn module of WU-BLAST 2.0 and an E-value cut off of 1 × 10^-5. The resulting outputs were filtered to exclude duplicates, and then assembled separately by species using CAP3 [49]. The total TPSs represents an estimated minimum number of unique TPSs found in each species.

ISSN: 1471-2229