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Figure 6 | BMC Plant Biology

Figure 6

From: Uncharacterized conserved motifs outside the HD-Zip domain in HD-Zip subfamily I transcription factors; a potential source of functional diversity

Figure 6

ATHB1 CTR acts as an activation domain in yeast cells. (A) The complete sequence of ATHB1, a version without the CTR (ATHB1WCT), and the CTR alone (ATH1CT) were fused to the DNA-binding domain of GAL4 (GAL4-BD). The empty vector expressing only the GAL4-BD was used as negative control. (B) A β-galactosidase activity assay. (C) Confirming this results, only the CTR and the complete ATHB1 protein had the transactivation activity required to reverse the auxotrophy to His of the AH109 yeast cells, allowing them to grow in medium lacking this amino acid.

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BMC Plant Biology

ISSN: 1471-2229

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