Figure 4

The effect of 3,4-DHP on the development and germination of banana somatic embryos and on the plant regeneration. Images (a), (c), (e) and (g) represent controls. Images (b), (d), (f) and (h) represent treatment with 200 μM of 3,4-DHP in RD1 embryo regeneration medium. (a) and (b) Embryo cultures one week after inoculation on RD1 medium showing many small black/necrotic cell aggregates resulting from 3,4-DHP treatment in the Figure (b). (c) and (d) Embryos 4 weeks after inoculation on RD1 embryo regeneration medium showing light brown embryos obtained on the embryo regeneration medium supplemented with 3,4-DHP in the Figure (d). (e) and (f) Embryo development 4 weeks after inoculation on RD2 medium for embryo maturation showing increased brown and black colour of 3,4-DHP treated plant material in the Figure (f). (g) and (h) Plant regeneration 4 weeks after inoculation on REG medium showing less regenerated plants after 3,4-DHP treatment in the Figure (h). Bars, 1 mm.