Developmental immunofluorescence localization of JIM11 epitope during somatic embryogenesis of banana. (a) Nonembryogenic cells showing signal located mainly at the surface of cell aggregates. (b) Detailed view from Figure (a) with arrowhead pointing on the moderate JIM11 signal at the surface of cell aggregates. (c) Embryogenic cells with strong signal, especially in the cell wall and cytoplasm around the nucleus but without signal on the surface of cell groups in many cases. (d) Detailed view from Figure (c) showing strong JIM11 signal in ECs. (e) Proembryos and globular embryos showing epidermis with strong surface fluorescence (arrow). (f) Detailed view from Figure (e) showing strong fluorescence in ECM covering epidermal cells (arrow). (g) Embryos at later stages. (h) Detailed view from Figure (g) showing strong signal in the tri-cellular junctions of cortical cells and moderate signal in the ECM at the surface (arrow). (i) and (j) Negative controls (labelled solely with secondary antibody) for ECs (i) and globular embryo (j). Bars, 100 μm.