Figure 7
From: Macromolecular composition of phloem exudate from white lupin (Lupinus albusL.)
Accumulation of miR399 in L. albus phloem exudate in response to Pi deficiency. Northern blot of 5 μg small RNA extracted from L. albus phloem exudate collected from plants that had been fertilised using a full nutrient solution (+P) or after 2, 3 and 4 weeks after Pi was omitted from the nutrient solution. RNA samples were separated on a 15% denaturing polyacrylamide gel, transferred to Hybond-N+ nylon membrane and hybridised to specific 32P end-labelled DNA oligonucleotide probes complementary to miR399. Low molecular weight RNA was visualized by ethidium bromide staining and serves as loading control.