Proteomic identification of OsCYP2, a rice cyclophilin that confers salt tolerance in rice (Oryza sativa L.) seedlings when overexpressed

Table 2 Identification of peptides from OsCYP2 (p8 protein spot) by MALDI-TOF-MS and ESI-MS/MS

Peptide no.	Match peptide sequences	Methods of identification		PercentCovered (%)^c	Modifications	Ion score	Ion score for acceptance
		MALDI-TOF MS	ESI-MS/MS
1	VFFDMTVGGAPAGR	+^a	+	8.14	None^d	64	38
2	TAENFR	+	-^b	3.49	None	ND^e	ND
3	TAENFRALCTGEK	+	-	7.56	None	ND	ND
4	GSTFHR	+	-	3.49	None	ND	ND
5	VIPEFMCQGGDFTR	+	+	8.14	Carbamidomethyl (C)	79	38
6	GNGTGGESIYGEK	+	-	7.56	None	ND	ND
7	GNGTGGESIYGEKFADEVFK	+	-	11.63	None	ND	ND
8	FADEVFK	+	-	4.07	None	ND	ND
9	HVVFGR	+	-	3.49	None	ND	ND
10	GGSTA KPV VIADCGQ LS	-	+	9.88	Carbamidomethyl (C)	48	38

^a +: positive match. ^b -: no match. ^c percent covered: percent of peptide sequence to OsCYP2 sequence. ^d None: without modifications. ^e ND: not determined.
^f Ion score for acceptance: individual ion score > 38 indicate identity or extensive homology (p < 0.05).

ISSN: 1471-2229