Figure 7

Expression of OsCYP2 in hybrid rice seedlings. (A) West blot showed expression of OsCYP2 in roots and shoots in 10-day-old rice seedlings. (B) RT-PCR showed time-course expression of OsCYP2 in seedlings of rice hybrid varieties, Shanyou 10 and Liangyoupeijiu, treated with 100 mM NaCl. (C) RT-PCR showed expression of OsCYP2 in hybrid seedlings under various stresses. Conc.: non-treated controls. Salt: 100 mM NaCl at 25°C for 3 h. PEG: 20% (w/v) PEG at 25°C for 3 h. Heat: 45°C for 3 h. Cold treatment: 4°C for 3 h. ABA: 50 mM ABA at 25°C for 3 h. Expression of OsCYP2 in hybrid rice seedlings was analyzed by RT-PCR. Actin (Os03g0718100) was used as an internal standard.