Amino acid sequence comparison of plant TLP-kinases (TLP-Ks). (A), Neighbour-joining tree of the 14 TLP-Ks identified in plants. Branch lengths are proportional to phylogenetic distances. Black star: sTLP-K from Brachypodium distachyon; grey star: TLP-K from Arabidopsis thaliana described in . (B), ClustalW amino acid alignment using the parameters described by  and manually adjusted. Thaumatin and protein kinase domains are respectively underlined in dark grey and black. Phobius  predicted transmembrane domain is underlined in light grey. Shaded boxes indicate highly conserved sequences. The arrow indicates the end of the predicted signal peptide. Vertical bars indicate cysteine residues in the thaumatin domain and aspartate residues forming the catalytic site of the kinase domain.