Figure 1

Visualization and sampling criteria for cotyledon pavement cells at different time points after germination. Fields of cotyledon epidermal cells stained with FM4-64 and subjected to simple morphometric analyses. Top row, left to right, fields of 2, 5, and 12 DAG cotyledon epidermal cells stained with FM4-64. Middle row: Same fields as top row showing the sampling scheme for cell measurements of complete cells that intersect a diagonal transect across the image field. Bottom row: example cells from each time point that were digitally dissected from the field, thresholded, and skeletonized. Bar = 100 μm