Figure 6
From: AtRabD2b and AtRabD2c have overlapping functions in pollen development and pollen tube growth
Pollen tube elongation defects in AtrabD2b , AtrabD2c and AtrabD2b/2c mutants. A, Pollen was germinated in vitro for 6 hours and examined by SEM. B, Germinated pollen was stained with aniline blue then observed under an epifluorescence microscope. C. Open flowers from an AtrabD2b/2c mutant plant, along with a wild-type plant, were incubated overnight on medium then examined by fluorescence microscopy. D. Close up view of pollen tubes in the AtrabD2b/2c mutant. E. Pollen was germinated in vitro and pollen tube length measured after an overnight incubation using SIS Pro software (OSIS, Lakewood, CO) (n > 200). Error bars indicate standard deviation. Scale bars = 10 μm (A); 50 μm (B, C); 20 μm (D).