Skip to main content
Figure 5 | BMC Plant Biology

Figure 5

From: Nickel and low CO2-controlled motility in Chlamydomonasthrough complementation of a paralyzed flagella mutant with chemically regulated promoters

Figure 5

Western blot of CYC6:RSP3-HA and CAH1:RSP3-HA transformants, probed with the anti-HA antibody. Panels A and B: Screening of protein extracts CYC6:RSP3-HA and CAH1:RSP3-HA transformants, extracted, respectively, 48 h and 6 h after induction. Transformants that exhibit inducible swimming are labeled. Arrows point at the RPS3-HA bands. Cultures were grown and induced as in Figures 3 and 4, extracted, and 20 μg total proteins were loaded on each lane. Panel C: Re-analysis of transformants exhibiting inducible swimming (from Panels A and B). Cultures were grown and induced as in Figure 6, extracted, and 40 μg total proteins were loaded on each lane. For details, see Methods.

Back to article page