Constitutive complementation of the pf14 mutant by the PSAD:RSP3-HA construct. Panel A: Percentage of swimming (S), flagellated-immotile (F/I) and non-flagellated (NF) cells in a single, rescued transformant. Panel B: Western blotting of the PSAD:RSP3-HA transformant and pf14 mutant probed with the anti-HA antibody. M, molecular weight marker. Cells were grown in 24-well microtiter plates. For details, see Methods.