Figure 6
In situ hybridization of WBLH transcripts in floral organs of CS (wild-type), CSdt7BS (normal flower) and (cr)-CSdt7BS (flower showing pistillody). (A) Transverse (left) and longitudinal (right) sections of a normal, fully developed pistil from CSdt7BS. The ovule is enveloped by an inner epidermis. The vascular bundle is connected to the chalaza. (B) In situ localization of WBLH1 transcripts in transverse sections of pistils of euplasmic and alloplasmic CS lines. (C) In situ localization of WBLH1 transcripts in longitudinal sections of the CS stamens. (D) In situ localization of WBLH2 transcripts in transverse sections of pistils and stamens of euplasmic and alloplasmic CS lines. Lower panels show higher magnification images of the pistils. The magnified image of CS was derived from another section for in situ hybridization analysis of WBLH2 transcript. (E) In situ localization of WBLH3 transcripts in longitudinal sections of developing florets from euplasmic and alloplasmic lines of CSdt7BS. St, stamen; Pi, pistil; tSt, transformed stamen; Ov, ovule; Ie, inner epidermis; Ch, chalaza; Vb, vascular bundle; Es, embryo sac; Mi, micropyle; Oi, outer integument; Ii, inner integument; Nu, nucellus; eOv, ectopic ovule. Scale bars represent 100 μm.