Figure 3

RT-PCR analysis of four BLH and three KNOX genes in the normal line (CS). Total RNA was isolated from roots, embryos on day 1 of germination, leaf blades, leaf sheaths, ligules and auricle-containing leaf regions, glumes, paleae, lemmas, stamens, pistils, young spikes (15-25 mm in length) and immature embryos 10 days after anthesis. The Act gene was used as an internal control. Number of PCR cycles is shown on the right of each panel.