Figure 1

Effects of NCS on the post-embryonic development of Arabidopsis roots. (A) Inhibitory effect of NCS on radicle protrusion during seed germination. (B) Wild type Arabidopsis seedling at 10 days after stratification grown on medium containing 0 or 5.0 μM NCS. Bar = 5 mm. (C) A close-up view of the whole plant in (B) treated with 5.0 μM NCS. Bar = 1 mm. (D) NCS inhibits primary root elongation. Root growth time course was measured after transferring 3-day-old Arabidopsis seedlings to NCS-containing medium. (E) NCS affects the lateral root density after exposing to NCS for 7 days. Data shown in (A), (D), (E) are means ± SD obtained from three independent experiments (n ≧ 20, P < 0.01). (F) Effects of NCS on cell expansion and cell division of meristematic cells. Arrows in the control panel showed the dividing cells. Bars = 50 μm in all panels. Differential interference contrast (DIC) images were captured using the transmission light detector of the confocal microscope.