Genetic mechanisms underlying the methylation level of anthocyanins in grape (Vitis viniferaL.)

BMC Plant Biology

Table 4 Substrate specificity of the recombinant AOMT2 proteins from Papadiko (PK-AOMT2) and from Petit-Bouschet × Aramon n°4 (PB-AOMT2)

Enzyme	Substrate	Products	relative amounts (%)
PK-AOMT2	Dp 3-glc	Dp 3-glc	51.1 (± 6.9) *
		Pt 3-glc	40.8 (± 4.0) *
		Mv 3-glc	8.1 (± 3.3)
	Cy 3-glc	Cy 3-glc	58.4 (± 12.2)
		Pn 3-glc	44.9 (± 15.4)
PB-AOMT2	Dp 3-glc	Dp 3-glc	63.6 (± 11.1) *
		Pt 3-glc	31.2 (± 8.6) *
		Mv 3-glc	5.3 (± 2.6)
	Cy 3-glc	Cy 3-glc	60.6 (± 9.3)
		Pn 3-glc	39.4 (± 9.2)

Reaction were essayed in vitro and products were identified according to their UV visible absorption spectra and retention time. Quantifications were based on peak areas at 520 nm. The data presented are the mean of 10 experiments performed with 3 independent protein preparations (± standard deviation), * indicates significance in ANOVA test (P-values < 0.05). Dp, delphinidin; Cy, cyanidin; Pt, petunidin; Pn, peonidin; Mv, malvidin; glc, glucoside

ISSN: 1471-2229