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Table 4 Substrate specificity of the recombinant AOMT2 proteins from Papadiko (PK-AOMT2) and from Petit-Bouschet × Aramon n°4 (PB-AOMT2)

From: Genetic mechanisms underlying the methylation level of anthocyanins in grape (Vitis viniferaL.)

Enzyme Substrate Products relative amounts (%)
PK-AOMT2 Dp 3-glc Dp 3-glc 51.1 (± 6.9) *
   Pt 3-glc 40.8 (± 4.0) *
   Mv 3-glc 8.1 (± 3.3)
  Cy 3-glc Cy 3-glc 58.4 (± 12.2)
   Pn 3-glc 44.9 (± 15.4)
PB-AOMT2 Dp 3-glc Dp 3-glc 63.6 (± 11.1) *
   Pt 3-glc 31.2 (± 8.6) *
   Mv 3-glc 5.3 (± 2.6)
  Cy 3-glc Cy 3-glc 60.6 (± 9.3)
   Pn 3-glc 39.4 (± 9.2)
  1. Reaction were essayed in vitro and products were identified according to their UV visible absorption spectra and retention time. Quantifications were based on peak areas at 520 nm. The data presented are the mean of 10 experiments performed with 3 independent protein preparations (± standard deviation), * indicates significance in ANOVA test (P-values < 0.05). Dp, delphinidin; Cy, cyanidin; Pt, petunidin; Pn, peonidin; Mv, malvidin; glc, glucoside