Figure 1

Confirmation of expression profiles by qRT-PCR experiment with 9 selected DEGs compared to microarray data in roots of rice NILs under 3 drought stress treatments with three biological replications for each stress condition. (A) Indicates comparison of microarray expression patterns of 9 selected DEGs from different functional categories with qRT-PCR data. In the histograms: × axis shows different rice genotypes used in this experiment i.e IR64 and four NILs including 10 = IR77298-14-1-2-B-10, 13 = IR77298-14-1-2-B-13, 11 = IR77298-5-6-B-11, and 18 = IR77298-5-6-B-18, severity of drought stress: FTSW0.2 indicates 20 percent of fraction of transpirable soil water which is considered as severe drought stress treatment, and FTSW0.5 indicates 50 percent of fraction of transpirable soil water which is considered as mild drought stress treatment., and Y axis representing fold changes in gene expression were transformed to log₂ scale, dotted lines indicate 1.5 fold or 1/1.5 fold, blue bar indicates the median of three qRT-PCR replicates, orange bar indicates the results of the median of three replications of microarray experiments. (B) Correlation analysis of the ratio of differentially expression level from microarray experiment to that from qRT-PCR at different drought stress treatments which are in good agreement with each other. The microarray data log₂-values (X-axis) were plotted against the qRT-PCR log₂-values (Y-axis). FTSW0.2 indicates 20 percent of fraction of transpirable soil water which is considered as severe drought stress treatment; FTSW0.5 indicates 50 percent of fraction of transpirable soil water which is considered as mild drought stress treatment and FTSW0.2+0.5 representing overall drought stress treatments.