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Table 1 Comparison of major EST sequencing efforts in common bean.

From: Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L.)

Sequence read

Full-length

Ramírez

Tibivilliers1

Clones

9,984

21,096

20,736

Sequence reads (after LQ & vector trimming)

7,079

15,781

37,919

Singletons

1,238

5,703

3,544

Contigs

2,981

2,266

7,510

Unigenes

4,219

7,969

10,581

Proportion of unigenes per sequence (%)

59.6%

50.5%

27.9%

Average EST length (nt)

563.8

606.2

656.4

Average contig length (nt)

677.9

606.2

1024.2

Average singleton length (nt)

568.3

594.7

691.7

  1. Two EST sequencing efforts are compared to the ESTs generated for the full-length cDNA libraries made in this study
  2. 1 clones from Thibivilliers et al. (2009) sequenced from both ends of the insert while those of Ramirez and this study were 5'end sequenced.
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BMC Plant Biology

ISSN: 1471-2229

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