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Table 1 Comparison of major EST sequencing efforts in common bean.

From: Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L.)

Sequence read Full-length Ramírez Tibivilliers1
Clones 9,984 21,096 20,736
Sequence reads (after LQ & vector trimming) 7,079 15,781 37,919
Singletons 1,238 5,703 3,544
Contigs 2,981 2,266 7,510
Unigenes 4,219 7,969 10,581
Proportion of unigenes per sequence (%) 59.6% 50.5% 27.9%
Average EST length (nt) 563.8 606.2 656.4
Average contig length (nt) 677.9 606.2 1024.2
Average singleton length (nt) 568.3 594.7 691.7
  1. Two EST sequencing efforts are compared to the ESTs generated for the full-length cDNA libraries made in this study
  2. 1 clones from Thibivilliers et al. (2009) sequenced from both ends of the insert while those of Ramirez and this study were 5'end sequenced.
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