Expression pattern of miRNA. miRNA expression levels from Solexa sequencing and qRT-PCR. Expression pattern of the selected 10 known miRNAs (miR156f, miR167d, miR169d, miR393a, miR394a, miR482, miR1507a, miR1508b, miR4369, and miR4397) measured by Solexa and qRT-PCR. 5s small RNA was used as a control in qRT-PCR. Total RNA (1 μl) from each of the four conditions (mock, drought, salinity and alkalinity) were used for verification.