EST sequencing and gene expression profiling of defence-related genes from Persea americana infected with Phytophthora cinnamomi

Table 6 Similarities between pyrosequencing data and gene expression profiles of defence-related genes

Sequence ID	GenBank accession number	cDNA Library	Max qRT-PCR expression	Similarities between 454 and qRT-PCR
Thaumatin	JO840464	Library 2	48 hpi	yes
LRR resistance PLP	JO840460	Library 1	12 hpi	yes
Metallothionein like protein	JO840461	Uninfected, Library 1	12 hpi	yes
Thaumatin-like protein	JO840465	Library 2	12 hpi	yes
Seven transmembrane protein MI0	JO840462	Library 1	12 hpi	yes
Pathogenesis-related protein PsemI	JO840463	Library 1	24 hpi	no
Proflin-like protein	JO840466	Library 2	12 hpi	no
Putative universal stress protein	JO840467	Library 2	12 hpi	no
Cytochrome P450 like TBP	JO840468	Library 1 and Library 2	3/12 hpi	yes

All the genes chosen for expression profiling from the tolerant avocado rootstock infected with Phytophthora cinnamomi showed the highest expression from a time point related to the cDNA library of their identification except the pathogenesis-related protein, profilin-like protein and the universal stress protein.

ISSN: 1471-2229