Figure 6
From: Differential expression of cysteine desulfurases in soybean
Cold stress effects on AO and XDH activity. (a) AO activity visualized by in situ staining after exposition of plants to cold stress for 18 h. Wells were loaded with 100 ÎĽg of protein of soybean wild type crude extracts of leaves from either untreated (1) or cold-stressed plants (2). Indole-3-carboxaldehyde plus 1-naphthaldehyde were used as substrate. (b) XDH activity visualized by in situ staining after exposition of plants to cold stress for 18 h. Wells were loaded with 100 ÎĽg of protein of soybean wild type crude extracts of leaves from either untreated (1) or cold-stressed plants (2). Hypoxanthine was used as substrate. (c) SDS PAGE gel 12% staining with Comassie blue. MW; molecular weight (Broad Range Protein Molecular Marked from Promega); wells were loaded with 100 ÎĽg of protein of soybean wild type crude extracts of leaves from either untreated (1) or cold-stressed plants (2).