Role of DOF-binding sites in the minimal promoter in driving GUS activity in guard cells. A, Schematic diagrams of constructs -262::GUS, -205::GUS and of constructs containing mutagenised version of the minimal promoter in different DOF-binding sites and in the W-box at position -234. B, Percentage of lines for each construct showing strong (column segment in black), intermediate (in dark grey), weak (light grey) or no signal (white). C, A leaf from a line harbouring the -262::GUS construct (a and a particular in e), shown as an example of strong GUS activity. In the following pictures examples of different lines harbouring the mDOF3::GUS construct showing respectively an intermediate (b and f), a weak (c and g) and no GUS activity (d and h). Scale bars represent 1 mm (a-d) or 0.1 mm (e-h).