High resolution melting analysis for the detection of EMS induced mutations in wheat Sbella genes

BMC Plant Biology

Table 6 Set of gene specific primer pairs used to produce TILLING 2^nd PCR amplicons.

Allele	Exon	primer forward (5'-3')	Primer Reverse (5'-3')	Size (bp)
	II	ccactgaccgactcact	atggacggggagattgg	215
	III	tcactattgtagtcatccttgcat	tgaagatttcccggcacg	156
	IV	tggtttcgttagtctgctct	tgagcgaaagtagcggg	313
	V	tttgggtatgcctccgt	tggaggcgcttcataatact	163
	VI	ttgctctaaatttatgatctggct	aggtggaagattgccaag	153
SBEIIa-A	VII	tgctcctattgatgccgat	gctacatgctcaactaaataattgg	152
	VIII	ctctgcccactaagggt	aaatttcatttaataatgtaatggagatcg	204
	IX	ccttttgtgaccatttactaaggata	accagaaacaggtgaaataact	157
	X	acaatacttagaggatgcatctga	ggtgaagaggcgcataca	212
	XI	ggtatttctgacttgtatgaccatt	accagataaacagtaaagcagc	223
	XII	gttgcattgcttcatcaatgatt	caaatatggtgacagaagtcagag	237
	XIII	tgttaaatctgttcttacacatgtcg	catagcaattatttcagtgccct	266
	IV	aacacactgctaaatttgaatgat	agactagtggaggcgtt	19
	V	tgctgaaggtatcatctaattgc	tgaccattaacaatagattagaaggtg	159
SBEIIa-B	VI	cagttactctaaatttatgatctggct	aggtggaagattgccaag	134
	VII	cctattgatgccgatatttgatatg	tcctcgactaaataattggccag	152
	VIII	aactctgcctactaagggt	acactggaaattccatttaataatgtaac	204
	IX	ccttttgtgaccatttactaaggata	ccggaaacaggtgaaataact	157
	II	actattgtagtcatccttgcatt	atgaagatttaccggcacg	157
	III	tcagtctgctctacaattgctat	gaaagcagcgggtaggc	301
	IV	gggtatacctcggtggattc	agactagtggaggcgttt	167
	V	gaaggtatcgtctaattgcatatct	caataaattggaaggtgtctcgtt	154
SBEIIa-D	IX	accatttactaaggatatttacatgcaa	accagaaacaggtgaaataact	151
	X	acaatacttagaggatgcatctga	ggtgaagaggcgcataca	212
	XI	ggtatttctgacttgtatgaccatt	accagataaacagtaaagcagc	223
	XII	gttgcattgcttcatcaatgatt	caaatatggtgacagaagtcagag	237
	XIII	tgttaaatctgttcttacacatgtcg	catagcaattatttcagtgccct	266

ISSN: 1471-2229