Skip to main content

Table 5 Set of genome specific primer pairs used to produce TILLING 1th PCR amplicons.

From: High resolution melting analysis for the detection of EMS induced mutations in wheat Sbella genes

Amplicon

Oligo-forward (5'-3')

Oligo-reverse (5'-3')

T. annealing

Size (bp)

A(II-V)

cgctcgctcgctccaatc

gcaactggtcagtattcagtaagctaag

65°C

1720

A(VI-IX)

tctgagaatatgctgggacgtag

gttcgaaaatgctacatgctca

62°C

1560

A(X-XIII)

ccagtggtcagaatgcatcaac

gggaactatctaagactccgtagcac

67°C

2100

B(IV-IX)

atgtggtggatgggttatgg

tccatagaataaaccatcagaccg

62°C

1970

D(II-VI)

atcgcgcttcctgaacctg

gggctgaagcttaagacactgac

65°C

1980

D(X-XIII)

gaggcagtgggcatgtgaaagtc

ctagggaactatctaagactccgtagcac

67°C

2200