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Table 3 Mutations affecting enzyme functionality as predicted by PARSE-SNP application.

From: High resolution melting analysis for the detection of EMS induced mutations in wheat Sbella genes

Gene

Nucleotide change

Mutation effect

PSMM diff.

 

G483A

G66D

32.06

SBEIIa-A

G485A

E67K

10.05

 

C1748T

P206S

16.09

 

C1755T

A208V

10.09

 

C2907T

Q301*

 
 

G5165A

W436*

 
 

G1916A

S. J.

 

SBEIIa-B

C1765T

A205V

14.06

 

G1948A

W220*

 
 

G511A

G62S

32.01

 

G520A

D65N

15.05

 

G1774A

A201T

13.08

SBEIIa-D

C3693T

Q346*

 
 

C3916T

H362Y

18.04

 

G3952A

G374R

22.08

 

G4000A

G390S

14.01

 

G4024A

V398I

14.07

 

G5278A

D462N

28.06

 

G5335A

S. J.

 
  1. The symbol "*" indicates nonsense mutations. S. J.= Splice Junction.