High resolution melting analysis for the detection of EMS induced mutations in wheat Sbella genes

BMC Plant Biology

Table 1 Overview of TILLING analysis.

Amplicon	Size (bp)	N° Plants analyzed	Mutations	Mutations density (kb per mutation)
A^(II-V)	493	2300	30	39
A^(VI-IX)	358	2688	26	40
A^(X-XIII)	498	1531	18*	34*
B^(IV-IX)	500	1152	14	40
D^(II-VI)	580	1920	23	31
D^(X-XII)	498	1920	30	33

*Mutations in amplicon A^(X-XIII)have not been characterized by sequencing with the exception of non sense mutation C2907T in exon X.

ISSN: 1471-2229