Figure 2
From: High resolution melting analysis for the detection of EMS induced mutations in wheat Sbella genes
High Resolution Melting analysis of second round PCR products of 96 2-fold pooled samples. The figure shows the analysis of the amplicon correspondent to exon VI of the SBEIIa-B gene. a) Total fluorescence (F) vs temperature (T) curves; b) comparison of dF/T curves between normal and heteroduplex (indicated by arrows) DNA amplicons; c) normalized and temperature-shifted curves of fluorescence vs temperature showing wild types (grey) and mutants (red); d) ΔF/T difference curves with variants highlighted in red.