Quantitative RT-PCR of RNA isolated from seed coat and cotyledon tissue at four stages of development. Each data point represents the average gene expression for two biological replicates, with three technical replicates for each biological replicate. Vertical bars represent one standard deviation. X-axis indicates days prior to seed maturity. Y-axis indicates gene expression relative to CONS6. 3A: qRT-PCR of R gene candidate Glyma09g36990, expressed as a relative measure of CONS6. 3B: qRT-PCR of anthocyanidin synthase gene expression (ANS, non-gene specific), relative to CONS6. 3C: qRT-PCR of UDP-glucose: flavonoid 3-O-glucosyltransferase (UF3GT, Glyma08g07130) gene expression, relative to CONS6.