Diagram of genetic mapping of the gene responsible for brown hilum in PI 283327. 2A: Diagram depicting the phenotype and allelic status of SSR markers within F6 RIL lines used to fine map the locus responsible for brown hilum color in soybean cultivar PI 283327. 2B: Screen capture of generic genome browser version 1.71, displaying the region identified which contained the locus responsible for the brown hilum color in soybean cultivar PI 283327(http://www.soybase.org, accessed 03-15-2011). Arrows indicate the location of the four candidate R2R3 MYB transcription factor genes. The genomic location of the only R2R3 MYB gene expressed in seed coats, which features a deletion from within exon 2 (C377-) in the brown hilum line (PI 283327) is indicated.