Graphical genotypes of recombinant plants selected from fine mapping and gene structure of γ-TMT3. (A). Summary of informative F5 plant lines used for fine mapping of the QTL responsible for high α-tocopherol concentration. Ichihime homozygous genotypes and KAS homozygous genotypes of each marker are represented by 'A' and 'B', respectively. Heterozygous genotype is represented by 'H'. '/' represent recombination positions. The region contributing to high α-tocopherol concentration is enclosed by a dashed box. KSC138-9 genotypes were only analyzed for these informative lines. The interval between KSC138-10 and KSC138-9 corresponded to a 75-kb sequence region on chromosome 9. Based on information from the Phytozome database, the region contained 10 predicted genes. Arrows referred to the genes and numbers below arrows correspond to the numbers in Table 2. (B). Gene structure of Glyma09g35680.1 (γ-TMT3). The green rectangles and the spaces between the green rectangles represent exons and introns, respectively. The yellow rectangle represents the 5'-UTR region, while the yellow arrow represents the 3'-UTR region. Vertical lines represent genetic polymorphisms (insertion-deletion, SNPs) between Ichihime and KAS. Nucleotide polymorphisms in the exons are indicated by vertical lines and numbers, which are summarized in Table 3. The polymorphisms numbered 2 and 4 are nonsynonymous nucleotide substitutions; the corresponding amino acid changes (Ichihime to KAS) are indicated below the substitution sites.