Schematic alignment of VIGS constructs and St genes and their closest off-target genes. Two sections of St1A were PCR amplified and ligated into VIGS plasmid pF13m. The identity of the constructs to each of the St genes is shown. E values for construct 1 and 2 against St and closest off target genes; St1A 2e-128, 2e-128; St 1B 4e-68, NA; St2A 2e-34 5e-48; St2B 1e-43 6e-60; St3A 2e-33 8e-27; St3B 3e-32 4e-24; Sb10g028730 3e-07, NA; Sb10g028720 3e-06, NA. NA indicates no significant similarity.