NDR1, NHL16 and NHL38 are the closest Arabidopsis relatives of CaNDR1 proteins. (a) Phylogenetic relationships between CaNDR1 proteins and their Arabidopsis relatives. The phylogenetic tree was built using the Phylowin freeware using the neighbor-joining method . Sequence alignment was previously obtained using version 2.0.10 of the Clustal X program . (b) Full length sequence alignment of CaNDR1a/b and the Arabidopsis protein NDR1, NHL16 and NHL38. Locations of the three NHL motifs within sequences are indicated with red lines above the alignment. The star indicates the amino acid residue substituted between both coffee NDR1 sequences. For sequence accession numbers, see legend of Figure 1.