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Figure 4 | BMC Plant Biology

Figure 4

From: The grapevine guard cell-related VvMYB60 transcription factor is involved in the regulation of stomatal activity and is differentially expressed in response to ABA and osmotic stress

Figure 4

The grape VvMYB60 gene complements stomatal defects of the Arabidopsis atmyb60-1 mutant. (A) and (B) Stomatal aperture assay in wild-type, atmyb60-1 and three independent transgenic mutant lines carrying the VvMYB30 (A) or the VvMYB60 (B) gene, under the control of the guard cell-specific AtMYB60 promoter. Measurements were performed on epidermal strips excised from dark-adapted plants and exposed to light for 4 hr. Each bar indicates mean ± SD of three separate experiments (n = 100 stomata per bar). The asterisk indicates values significantly different from wild-type (P < 0.001, t-test). (C) and (D) Changes in soil water content during drought stress treatment of wild-type, atmyb60-1 and three independent mutant lines complemented with the VvMYB30 gene (C), or the VvMYB60 gene (D). Plants grown under normal watering conditions for 20 days were drought stressed by complete termination of irrigation. For clarity the responses of the atmyb60-C30 and atmyb60-C60 transgenic lines have been plotted in two different graphs. Each point indicates mean ± SD (n = 20).

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