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Figure 2 | BMC Plant Biology

Figure 2

From: The grapevine guard cell-related VvMYB60 transcription factor is involved in the regulation of stomatal activity and is differentially expressed in response to ABA and osmotic stress

Figure 2

Gene expression profiles of VvMYB60 and VvMYB30 in different plant tissues and in response to ABA. (A) Expression in grapevine organs. (B) and (C) Expression throughout berry seed and skin development (X-axis corresponds to weeks from veraison). Each gene was independently normalized. (D) and (E) Expression in response to applied ABA in leaf disks. X-axis corresponds to hours after ABA application. White circle: Mock solution, black circle: 50 μM ABA, black triangle: 100 μM ABA. (F) Expression changes of VvMYB60 and VvMYB30 in grapevine plantlets subjected to salt stress conditions. Each gene was independently normalized against its control treatment (standard MS, with 3 mM NaCl). Means and SD are the result of three independent replicates. Reference genes (UBIQUITIN and GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE) were differently selected according to the experimental condition in which they presented less variation among samples and assuming they behaved similarly as described in [43].

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