Figure 7
From: Nodulin 41, a novel late nodulin of common bean with peptidase activity
PvNod41 is a late nodulin. (A) 12% SDS-PAGE analysis of crude protein extracts from roots and root nodules. Lane 1 and 12, crude protein extracts from 3-d-old uninoculated roots and 21 days post inoculation (dpi) nodule-stripped roots, respectively. Lanes 2 to 11, crude extracts from 10 (lane 2), 12 (lane 3), 14 (lane 4), 16 (lane 5), 18 (lane 6), 20 (lane 7), 22 (lane 8), 25 (lane 9), 27 (lane 10) and 30 (lane 11) dpi root nodules. Arrowhead indicates the accumulation of leghemoglobin during nodule ontogeny. (B) Western blot analysis of the same samples using the anti-PvNod41 antiserum. (C) Accumulation of PvNod41 transcripts during nodulation. Equivalent samples to A and B were analyzed by RT-qPCR to determine PvNod41 gene expression levels. Eight technical replicates were analyzed per sample. Error bars represent the standard error.