Figure 5
From: Nodulin 41, a novel late nodulin of common bean with peptidase activity
Effect of pH on the activity of PvNod41. (A) Purified PvNod41 was tested for activity using casein as a substrate (1 h at 37°C) at pH values ranging from 2.5 to 9.5. Obtained samples were analyzed by 12% SDS-PAGE and stained with Coomassie Blue. (B) Densitometry analysis of degraded casein. Percentage (%) of degraded casein relative to control casein was plotted against pH. Means of three independent experiments ± SE are shown.