Figure 2
From: Nodulin 41, a novel late nodulin of common bean with peptidase activity
PvNod41 primary sequence. PvNod41 gene sequence (lower case) and protein sequence (upper case). PvNod41 encodes a 437 amino acid single polypeptide containing Asp-Thr-Gly and Asp-Ser-Gly sequences (DTG and DSG). Conserved motifs around the two catalytic aspartic acid residues are shown in boldface and underlined. Primer sequences used for PCR amplification are underlined. The arrow indicates the cleavage position of the putative signal peptide that directs the protein to the ER. HPLC-purified peptide sequences obtained from the trypsin digestion of PvNod41 [N-terminal end (N-term) as well as three internal peptides (P-1, P-2 and P-3)] are also depicted in this figure. The stop codon is marked with an asterisk.