Complex formation of Tic20 in inner envelope membranes and proteoliposomes. Two-dimensional BN/SDS-PAGE of (A) inner envelope vesicles of Pisum sativum (Ps, 100 μg protein) and (B) AtTic20-proteoliposomes (20-30 μg protein). Samples were solubilized in 5% digitonin and separated by 4-13% BN-PAGE followed by 12.5% SDS-PAGE. Indicated specific antibodies were used for immunodetection. Representative results are depicted. At - Arabidopsis thaliana.