Effects of calcium ions on electrophoretic mobility of olive pollen caleosin. Effects of calcium ions on electrophoretic mobility of caleosin isolated from pollen OBs (A) and pollen coat (B). Proteins were extracted from pollen OBs and pollen coat and pre-treated with EGTA. Ten μg of proteins were incubated with CaCl
(left lane), CaCl
followed by EGTA (middle lane), or both CaCl
and EGTA simultaneously (right lane). Immunoblots were probed with a FL anti-Clo3 Ab, followed by an anti-rabbit IgG Alexa 633-conjugated secondary Ab. Arrows show caleosin bands. Protein markers (kDa) are indicated on the left.