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Table 2 RT-qPCR expression analysis of 32 stress-related genes showing significant differential expression in tissues from sugarcane variety Q117.

From: Identification of drought-response genes and a study of their expression during sucrose accumulation and water deficit in sugarcane culms

Gene Mean
  P value LFE1 I 4-5 I 7-8 I 13-14
Up-regulated Trehalase <.001 0.0102a 0.0230b 0.0240b 0.0370c
TAP24F-4 <.001 0.1241a 1.1617c 0.7071b 0.6160b
TM89-33 0.002 0.0659a 0.1502c 0.1059b 0.1418c
TF1 2 0.009 0.0395 a 0.1370 b 0.1238 b 0.1866 b
TM11b 0.0342 0.0105a 0.0217b 0.0195ab 0.0250b
Pox <.001 0.1511 ab 0.1127 a 0.1879 b 0.2798 c
OAT <.001 0.0205 a 0.0199 a 0.0201 a 0.0872 b
AS <.001 0.0126 a 0.1024 b 0.1159 b 0.1591 c
Samsynt <.001 1.9011a 11.9409b 10.8635b 13.0849b
SPDS 0.0108 0.0785a 0.0744a 0.0935ab 0.1056b
PST2a <.001 0.0566a 3.2513b 3.4418b 2.5815b
PST2b 0.008 0.0369a 0.1816b 0.2064b 0.1904b
PST5 0.021 0.0141 a 0.0688 ab 0.1428 bc 0.2272 c
PST7 <.001 0.0577a 0.2210c 0.1330b 0.1302b
LEA <.001 0.0415 a 0.0755 a 0.0826 a 0.3197 b
Dehydrin 0.0442 0.0074 a 0.0158 a 0.0088 a 0.0454 b
ABC transporter 0.025 0.0252a 0.0490ab 0.0408a 0.0665b
P5CS 0.056 0.1093 a 0.1424 a 0.1465 a 0.2511 a
Down-regulated Gols 0.009 0.1009b 0.0571a 0.0172a 0.0092a
TPP 0.003 0.8318b 0.4294a 0.3904a 0.3016a
DREB-like protein 0.02 0.0016a 0.0057b 0.0022a 0.0019a
THB43-11 <.001 0.3665b 0.1105a 0.1361a 0.1502a
HvDRF1 <.001 0.4607c 0.2112b 0.1223a 0.1409a
TWC1 <.001 0.0529b 0.0280a 0.0200a 0.0206a
ShSUT1 0.001 0.1434b 0.1261b 0.0697a 0.0481a
DnaJ <.001 0.2349a 0.9842c 0.6780b 0.4610ab
HPPase <.001 0.0105a 0.3023b 0.0350a 0.0270a
Osmotin 0.003 0.0503a 0.2302b 0.0572a 0.0577a
Stress-related protein 0.002 0.0997a 0.3784b 0.1460a 0.1152a
Expansin <.001 0.3810a 4.1719b 0.1148a 0.0389a
Lipoxigenase <.001 0.0013b 0.0002a 0.0002a 0.0003a
PEAMT 0.016 0.2207b 0.2079b 0.0979a 0.0926a
ADI <.001 0.0153a 0.0251c 0.0194b 0.0181ab
  1. 1The tissues are as described in Table 1. The expression of each gene was normalised relative to that of GAPDH.
  2. 2The eight genes selected for analysis in further experiments are shown in bold typeface. (See additional file 2 for full name of each gene and primers sequences). Data for PC5S (P = 0.056) which was included in further experiments (see text) is also presented.
  3. aMean values within a gene with the same letter are not significantly different based on LSD test (P ≥ 0.05) from one-way analysis of variance (ANOVA).