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Figure 3 | BMC Plant Biology

Figure 3

From: A single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters selectivity

Figure 3

Unlike VvMYB5bR, VvMYB5bL is not able to activate VvCHI promoter in grape cells. Results of transient expression after co-bombardments of cultured grape cells with the Firefly luciferase reporter gene fused to the VvCHI promoter and combinations of VvMYB5bR or VvMYB5bL, together with AtEGL3 and AtTTG1. The normalized luciferase activity was calculated as the ratio between the Firefly and the Renilla luciferase (used as internal control) activity [63]. All bombardments included the WD40 protein AtTTG1 (GenBank accession number AJ133743). Values indicate the fold increase relative to the activity of the VvCHI promoter transfected without transcription factors. Each column represents the mean value ±SD of three independent experiments (Student's t test; * P < 0.05 vs. VvCHI alone).

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