Figure 2

The single residue substitution R69L reduces VvMYB5b trans-activation capacity in yeast. VvMYB5b^R and VvMYB5b^L coding sequences were fused to GAL4 DNA Binding Domain (DBD) and their ability to activate LacZ reporter gene expression was quantified using β-galactosidase activity measurements. Each value is the mean ±SD of two independent yeast transformations and each experiment included three measures (Student's t test; * P < 0,05 vs. negative control). Constructs are identified as indicated to the left of the figure. MEL1 UAS, Melibiose 1-GAL4 Upstream Activating Sequence; mp, minimal promoter; pADH1, Alcohol Dehydrogenase 1 promoter. Both MYB repetitions (i.e. R2 and R3 repeats) are indicated using dashed boxes.