Figure 2

Representation of cloning of Rpi-vnt1.3 using Sol R gene. A) Resistant Solanum germplasm is selected based on screenings with P. infestans isolates. Results to isolate IPO-C are presented. Graphic representation facilitates quick overview of the quantitative resistance data: the red indicator shows the resistance level that ranges from fully susceptible (0, left) to fully resistant (9, right). B) Resistant genotypes (365_1) are crossed with related susceptible genotypes (735_2), selected from the phylogenetic tree. C) Population 7663 (365_1 × 735_2) is segregating for resistance to P. infestans isolate IPO-C, which is visualized by the frequency distribution for the offspring. The progeny part that contains Rpi-vnt1.3 is highly resistant (right bar, resistance level 8), whereas the progeny that lacks Rpi-vnt1.3 is moderately susceptible (left bar, resistance level 3). D) After genetic mapping, the Rpi-vnt1.3 was cloned, and used for allele-mining. In this menu, R genes and related sequences can be retrieved.