Figure 3
From: Fusarium graminearumforms mycotoxin producing infection structures on wheat
Quantification of deoxynivalenol (DON) in glumes and caryopses infected with F. graminearum TRI5prom::GFP at 8 dpi. 28 infected glumes and 28 caryopses of the wheat cultivar Nandu were pooled for the estimation of DON after GFP-fluorescence of the fungus was visible by fluorescence microscope MZFLIII. The concentration of DON in ppb of infected fresh weight was estimated by the RIDASCREEN DON enzymatic immunoassay (R-Biopharm, Darmstadt, Germany). Three replicative measurements were done for statistical analyses. Mock inoculated glumes were used as a negative control.