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Figure 7 | BMC Plant Biology

Figure 7

From: Harpin-induced expression and transgenic overexpression of the phloem protein gene AtPP2-A1 in Arabidopsis repress phloem feeding of the green peach aphid Myzus persicae

Figure 7

Genetic construction used in generation of uidAETAt ( AtPP2-A1-promoter-uidA- expressing A. thaliana ) and organ-unspecific uidA expression in uidAETAt. (a) The construct. The AtPP2-A1 promoter (PP2P) was inserted into the binary vector pBI121 at the Hind III and BamH I restriction sites to replace 35S while reserve the reporter gene uidA encoding β-D-glucuronidase (GUS). Labels are the same as in Figure 5a. (b) Appearance of 35-day-old plants of the uidAETAt1 line compared with the transgenic control plant. (c, d) RT-PCR analysis of uidA expression and GUS activity in different organs of 35-day-old uidAETAt1 compared with the transgenic control plant.

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BMC Plant Biology

ISSN: 1471-2229

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