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Figure 3 | BMC Plant Biology

Figure 3

From: The Arabidopsis translocator protein (AtTSPO) is regulated at multiple levels in response to salt stress and perturbations in tetrapyrrole metabolism

Figure 3

Stress-response genes are up-regulated in tspo-1 during salt stress. (A)-(C) Stress-induced gene expression in OxM1TSPO:eGFP and tspo-1 lines compared to wild type plants, by qPCR. 5-day-old seedlings grown under standard conditions and transferred for 3 hours to plates containing 150 mM NaCl. (A) DREB2A, (B) RAB18 and (C) ERD10 mRNA levels were determined by quantitative qRT-PCR. Relative amounts were calculated and normalized relative to Col-0 non-treated (100%). The ACTIN and 18S rRNA were used as reference genes. ACTIN, At3g18780; 18S RNA, At3g41768; RAB18, At5g66400; ERD10, At1g20450; DREB2A, At5g05410. Data shown represent mean values obtained from independent amplification reactions (n = 3) and biological replicates (n = 2). Relative expression levels were calculated. Bars represent the standard error of biological replicates.

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