Figure 6

Progressive Z-stack series of a single cell, illustrating mitochondria and chloroplasts associations with transvacuolar strands within a lace plant window stage leaf. A z-stack progression consisting of four focal planes within one CMXRos stained cell in the center of a window stage leaf areole. Red fluorescence represents mitochondria while green fluorescence represents chlorophyll autofluorescence. A) and B) DIC and corresponding fluorescent images, respectively, in the top most plane of the cell. Note the mitochondria and chloroplasts around the nucleus. C) and D) DIC and corresponding fluorescent images, respectively in a lower focal plane. E) and F) DIC and corresponding fluorescent images, respectively in a middle focal plane within the cell. Note the continued association of chloroplasts and mitochondria around the nucleus, and the appearance of a strand in the lower right hand corner of the cell. G) and H) DIC and corresponding fluorescent images, respectively, displaying the lower most focal plane within this cell. Note the transvacuolar strand, which appears to have CMXRos stained mitochondria associated with it. Scale bars (A-H) = 25 μm.