Figure 5

TUNEL assay portraying TUNEL-positive nuclei within a single areole of a stage 2 or window stage leaf. TUNEL-positive nuclei within a single areole of a stage 2 (window stage) leaf. Note that Propidium Iodide (PI) staining is red, TUNEL-positive nuclei stain green and when red and green nuclei overlap they appear yellow. A) Low magnification differential interference contrast (DIC) image of a portion of a single areole in a window stage leaf B) Corresponding low magnification TUNEL-positive image C) corresponding low magnification PI image D) overlay of TUNEL-positive and PI images. E-H) High magnification images taken of NPCD cells where stage M1 mitochondria are normally found, DIC, TUNEL assay, PI and overlay of all three respectively. I-L) High magnification images taken of EPCD cells where stage M2 mitochondria are normally found, DIC, TUNEL assay, PI and overlay of all three respectively. M-P) High magnification images taken of LPCD cells where stage M3 mitochondria are normally found, DIC, TUNEL assay, PI and overlay of all three respectively. Q-T) High magnification images taken of LPCD cells where stage M4 mitochondria are normally found, DIC, TUNEL assay, PI and overlay of all three respectively. Scale bars (A-D) = 60 μm; (E-T) = 15 μm.