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Figure 3 | BMC Plant Biology

Figure 3

From: Do mitochondria play a role in remodelling lace plant leaves during programmed cell death?

Figure 3

Mitochondrial distribution (stage M1-M4) within a window stage lace plant leaf. Mitochondria within a window stage leaf stained with CMXRos and examined via confocal microscopy to view organelle distribution throughout the PCD gradient within individual cells. A) and B) Stage M1 DIC and corresponding CMXRos fluorescent images, respectively. C) TEM micrograph of healthy mitochondria depicting intact mitochondrial membranes and cristae. D) and E) Stage M2 DIC and corresponding CMXRos fluorescent images, respectively. Note mitochondria most have aggregated within the cell with several individual mitochondria still present in the cytosol. F) TEM micrograph of mitochondria within dying cell depicting what appears to be a healthy mitochondria with intact cristae and clear membranes. G) and H) Stage M3 DIC and corresponding CMXRos fluorescent images, respectively. Mitochondria are still aggregated within the cell. I) TEM micrograph of degrading mitochondria, mitochondrial cristae appear to be degraded, with less clear inner and outer membranes as compared to controls. J) and K) Stage M4 DIC and corresponding CMXRos fluorescent images, respectively. Note mitochondria have lost membrane potential entirely and are no longer visible in the fluorescent image. Mitochondria are now considered un-viable. L) TEM micrograph of presumably dead mitochondria depicting nearly indistinguishable membranes and damaged cristae. Scale bars (A, B, D, E, G, H, J, K) = 10 μm; (C, F, I, L) = 0.5 μm

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