Change in transcriptome associated with flowering and grain filling . (A) Expression pattern of 29,119 genes from 62 to 125 DAT based on relative expression values. Transient expression of pollen specific genes at 90 DAT (indicated by asterisk), which also coincided with the peak of flowering, was due to pollen contamination of leaf samples (See Additional file 15). (B) PCA of the expression profile of flag leaf at 83, 90, 97 and 104 DAT. (C) Cluster analysis of fertile (F) and sterile (S) plants at 0, 1, 2 and 3 weeks after heading (WAH) based on correlation distance and complete linkage. (D) Differentially expressed genes in flag leaf of fertile (blue) and sterile (red) lines after heading were statistically extracted based on t-test and fold change criteria (FDR < 0.05 and FC > 2).